Platelets

Structure of platelets

  • Small (0.5-3Ξm) irregular anucleate cells
  • Contain:
    • mitochondria, ATP and glycogen
    • Dark (dense) granules
      • ADP, ATP, calcium, serotonin
    • alpha granules
      • VWF, fibrinogen, clotting factor V, XIII
    • lysosomes
      • Microtubules and surface-connected canaliculi
  • External glycocalyx layer (thick, 20-30nm)
  • Released as fragments of megakaryocytes
  • Regulated by thrombopoietin
  • Destroyed by Kupffer cells in the liver + spleen
  • about ⅓ in spleen at any one time

Function of platelets

  • Adhesion to the denuded surface collagen via VWF, as well as directly
  • Aggregation (platelet to platelet) mediated by fibrin and VWF → platelet plug
  • Activation, which means
    • Degranulation (release of vasoactive and platelet-activating mediators)
      • Thromboxane A2 → ðŸ’Ķ 5HT, ADP release → vasoconstriction etc
      • +ve feedback loop
    • Shape change (flattening and extension of cellular projections)
    • Phosphatidylserine exposure on the platelet surface, which is essential for clotting factor binding

Factors that oppose platelet activity

  • Prostacyclin (see Epoprostenol)
  • Nitric oxide
  • Fibrin degradation product